Ciliobrevin D is primarily used in research settings to study the role of dynein, a motor protein involved in intracellular transport. It acts as an inhibitor of dynein, allowing scientists to investigate the protein's function in cellular processes such as mitosis, organelle positioning, and vesicle transport. By disrupting dynein activity, researchers can better understand its contribution to cell division, neuronal function, and ciliary movement. This compound is particularly valuable in studies related to developmental biology, neurobiology, and diseases linked to dynein dysfunction, such as neurodegenerative disorders. Its application helps elucidate the mechanisms of intracellular transport and provides insights into potential therapeutic targets.