Used as a fluorescent probe in biological imaging due to its strong emission upon binding to specific protein targets. It selectively binds to misfolded protein aggregates, making it valuable in research on neurodegenerative diseases such as Alzheimer's and Parkinson’s. The compound’s (E)-configuration enhances its photostability and quantum yield, allowing for prolonged live-cell imaging with minimal photobleaching. Also employed in high-throughput screening assays to identify inhibitors of protein aggregation. Its nitrile groups facilitate interactions with hydrophobic pockets in amyloid fibrils, improving binding affinity and specificity.