β-Secretase Inhibitor II is primarily used in research to study the role of β-secretase in the production of amyloid-beta peptides, which are implicated in the pathogenesis of Alzheimer's disease. It is a valuable tool for investigating the mechanisms of amyloid plaque formation and for evaluating potential therapeutic strategies targeting β-secretase activity. Additionally, this inhibitor aids in understanding the biochemical pathways involved in neurodegenerative disorders and supports the development of drugs aimed at reducing amyloid-beta accumulation in the brain.