FGIN-1-27 is primarily utilized in research settings to study the role of translocator protein (TSPO) in various biological processes. It is commonly applied in neuroscience to investigate its effects on neurosteroid synthesis, as TSPO is involved in the regulation of neurosteroid production, which can influence anxiety, stress, and other neurological conditions. Additionally, FGIN-1-27 is used in studies exploring mitochondrial function and cellular energy metabolism, given TSPO's presence in mitochondrial membranes. It also serves as a tool compound in pharmacological research to understand the therapeutic potential of TSPO modulation in conditions like inflammation, neurodegenerative diseases, and cancer. Its applications are largely confined to experimental and preclinical studies, contributing to the development of novel therapeutic strategies.