Aflatoxin B2 is primarily used in research settings as a reference standard for detecting and quantifying aflatoxin contamination in food and agricultural products. It serves as a critical tool in food safety laboratories to calibrate analytical instruments such as HPLC and mass spectrometers. Due to its known mutagenic and carcinogenic properties, it is also employed in toxicological studies to assess the health impacts of long-term exposure to aflatoxins, particularly on liver function and DNA damage. Additionally, it plays a role in evaluating the effectiveness of detoxification methods and storage practices aimed at reducing mycotoxin levels in crops like corn, peanuts, and tree nuts. Its use is strictly controlled due to high toxicity and regulatory concerns.