Aflatoxin B2-d3 is primarily used as an internal standard in analytical procedures for the detection and quantification of aflatoxins in various samples. Its deuterated structure ensures accurate and reliable measurements by compensating for potential losses during sample preparation and analysis. This compound is essential in food safety testing, particularly in monitoring contamination levels in agricultural products like grains, nuts, and spices. It also plays a critical role in research studies focused on understanding the metabolism and toxicological effects of aflatoxins, aiding in the development of strategies to mitigate their impact on human and animal health.