Pyroligneous acid, also known as wood vinegar, is used as a natural wood preservative due to its antimicrobial and antifungal properties. It is commonly applied in traditional woodworking and furniture making to protect untreated wood from decay and insect damage, as well as to darken or age the wood for aesthetic enhancement, adding depth and visual appeal suitable for crafts and decorations.

In organic agriculture, when properly diluted, it serves as a soil conditioner, disinfectant to inhibit fungi, and plant growth stimulant.

In the food industry, after distillation to remove impurities and toxins, it is used as a flavoring agent in smoked foods to impart a smoky taste and extend shelf life.

Additionally, it is utilized in eco-friendly cleaning products for deodorizing and disinfecting surfaces, owing to its mild acidity and phenolic compounds that provide antimicrobial effects.