Pimpinellin is primarily known for its role in plant defense mechanisms and has shown potential in agricultural applications as a natural pesticide. It exhibits antifungal and antibacterial properties, helping protect plants from microbial pathogens. Due to its presence in certain essential oils and aromatic plants, it contributes to the overall bioactivity of plant extracts used in organic farming. In pharmacological research, Pimpinellin demonstrates anti-inflammatory and antioxidant activities, making it a candidate for development in natural health products. It has also been studied for its ability to modulate enzyme activity, particularly in relation to drug metabolism, which suggests possible interactions with other bioactive compounds in medicinal contexts. Additionally, Pimpinellin is investigated for its allelopathic effects—its ability to influence the growth of neighboring plants—offering insights into natural weed control strategies in sustainable agriculture. Its low toxicity and natural origin support interest in eco-friendly applications across agrochemical and pharmaceutical fields.