Dehydroherbarin exhibits significant biological activity, making it valuable in pharmaceutical and agricultural applications. It demonstrates strong antifungal properties, particularly against plant pathogens, suggesting its potential use as a natural fungicide in crop protection. Its ability to inhibit microbial growth extends to certain Gram-positive bacteria, indicating possible utility in developing antimicrobial agents.

In medicinal research, Dehydroherbarin shows anti-inflammatory and cytotoxic effects, which are being explored for drug development, especially in oncology and immunology. It also acts as a secondary metabolite with signaling properties in fungal systems, useful in studying microbial interactions and ecological relationships. Due to its natural origin and bioactivity, it is of interest in green chemistry and sustainable agrochemical design.