Blumenol B 9-O-glucoside is primarily used as a biomarker for arbuscular mycorrhizal (AM) fungal activity in plants. It accumulates in plant tissues in response to symbiotic colonization by AM fungi, making it a valuable indicator in studies of plant-microbe interactions. Researchers use this compound to assess the extent of mycorrhizal colonization without destructive sampling, especially in root systems. It is also employed in ecological and agricultural research to evaluate soil health, plant nutrient uptake efficiency, and the impact of environmental stress on symbiotic relationships. Due to its specificity and stability, it supports non-invasive monitoring of belowground processes in both natural and cultivated ecosystems.