Used as a fluorescent probe in biochemical research to study protein conformation and interactions. It binds to hydrophobic regions of proteins, allowing for the detection of structural changes through fluorescence spectroscopy. Commonly applied in assays to monitor protein folding, unfolding, and aggregation. Also utilized in the analysis of membrane proteins and lipid bilayers due to its sensitivity to environmental polarity. Additionally, it serves as a tool in studying enzyme kinetics and molecular dynamics in various biological systems.