Used as a fluorescent probe in biochemistry and molecular biology to study protein conformation and interactions. It binds to hydrophobic regions of proteins, enhancing fluorescence and allowing detection of structural changes. Commonly applied in assays to monitor protein unfolding, folding, and ligand binding. Also utilized in membrane studies to investigate lipid bilayer properties and dynamics. Its sensitivity to environmental changes makes it valuable for analyzing macromolecular interactions and structural transitions in various biological systems.