Widely used as a fluorescent probe in biochemical and biophysical research, this compound is particularly effective in detecting conformational changes in proteins and monitoring molecular interactions. It exhibits enhanced fluorescence when bound to hydrophobic regions of proteins, making it a valuable tool for studying protein folding, denaturation, and aggregation. Commonly employed in assays involving amyloid formation, it helps identify misfolded protein structures associated with neurodegenerative diseases. Also utilized in micelle formation studies and membrane mimic systems due to its sensitivity to environmental polarity. Its strong signal response upon binding to non-polar surfaces allows for precise detection of binding events in solution-based experiments.