Widely used as a fluorescent probe in biochemical and cellular assays due to its strong emission properties in the visible range. It is particularly effective in labeling biomolecules such as proteins and nucleic acids, enabling detection and imaging in fluorescence microscopy. Commonly employed in enzyme activity assays, especially for proteases and esterases, where it acts as a quenched or turn-on fluorescent substrate. Its hydrazide group allows conjugation with carbonyl-containing compounds, making it useful in the development of sensors for aldehydes and ketones in biological samples. Also utilized in time-resolved fluorescence studies and immunoassays due to its photostability and sensitivity.