ML241 hydrochloride is primarily used in research settings as a selective inhibitor of the enzyme N-acylethanolamine acid amidase (NAAA). This enzyme plays a role in the breakdown of bioactive lipids, particularly palmitoylethanolamide (PEA), which is involved in regulating inflammation and pain. By inhibiting NAAA, ML241 hydrochloride enhances the levels of PEA, making it a valuable tool for studying inflammatory and pain-related pathways. Researchers utilize this compound to explore potential therapeutic strategies for conditions such as chronic pain, neuroinflammation, and other disorders linked to lipid signaling. Its specificity and potency make it a key compound in preclinical studies aimed at understanding the biological mechanisms of NAAA and its implications in disease.