(S)-3'-amino Blebbistatin is primarily used in biological research as a selective inhibitor of myosin II, a motor protein involved in muscle contraction and various cellular processes. Its application is particularly valuable in studies focused on understanding the role of myosin II in cell motility, cytokinesis, and cellular mechanics. Researchers utilize this compound to investigate the dynamics of actin-myosin interactions and their impact on cell behavior, such as migration, division, and tissue organization. Additionally, (S)-3'-amino Blebbistatin is employed in live-cell imaging experiments due to its reduced phototoxicity compared to other myosin inhibitors, making it suitable for prolonged observation of cellular processes. It also aids in exploring the mechanisms of diseases related to myosin dysfunction, such as certain types of cancer and cardiovascular disorders, providing insights for potential therapeutic strategies.