(S)-3'-hydroxy Blebbistatin is primarily utilized in biological research to study cellular processes involving myosin II, a motor protein essential for muscle contraction and various cellular movements. It acts as a selective inhibitor of myosin II ATPase activity, allowing researchers to investigate the role of myosin II in cell division, migration, and cytoskeletal organization. Its application is particularly valuable in understanding cell mechanics and dynamics in fields such as cancer research, where myosin II plays a role in tumor cell invasion and metastasis. Additionally, it is used in developmental biology to explore the mechanisms of tissue morphogenesis and organ formation. The compound’s ability to reversibly inhibit myosin II makes it a useful tool for studying these processes without causing permanent damage to cells.