The Probe-quantitative Real-time PCR Kit for Mycoplasma mycoides subsp. mycoides is primarily used in veterinary diagnostics and research to detect and quantify the presence of this specific pathogen. It is essential for monitoring and controlling contagious bovine pleuropneumonia (CBPP), a severe respiratory disease in cattle caused by this bacterium. The kit enables rapid, accurate, and sensitive detection in clinical samples, such as nasal swabs, lung tissue, or blood, facilitating early diagnosis and effective disease management. It is also valuable in epidemiological studies to track outbreaks and assess the effectiveness of vaccination programs. Additionally, the kit supports regulatory compliance in livestock trade by ensuring animals are free from infection before transportation. Its high specificity minimizes false positives, making it a reliable tool for both field and laboratory use.