4-DAMP is primarily used in pharmacological research as a selective antagonist for muscarinic acetylcholine receptors, particularly the M3 subtype. It is widely employed in studies investigating the role of these receptors in various physiological processes, such as smooth muscle contraction, glandular secretion, and neurotransmission. In experimental settings, 4-DAMP helps to elucidate the mechanisms of action of acetylcholine and other muscarinic agonists by blocking M3 receptor activity. This makes it a valuable tool in understanding disorders related to muscarinic receptor dysfunction, such as asthma, overactive bladder, and gastrointestinal motility disorders. Additionally, it is used in the development and testing of therapeutic agents targeting muscarinic receptors.