Oxotremorine M iodide is primarily used in research settings to study the cholinergic system, particularly in neuroscience and pharmacology. It acts as a muscarinic acetylcholine receptor agonist, making it a valuable tool for investigating the role of these receptors in various physiological processes. Researchers utilize it to explore the effects of muscarinic receptor activation on memory, learning, and cognitive functions. It is also employed in studies related to neurodegenerative diseases, such as Alzheimer's, to understand the involvement of cholinergic signaling in disease progression. Additionally, it is used in experimental models to induce tremors, helping to study the mechanisms of motor control and the potential therapeutic effects of anti-tremor agents. Its application extends to the development of drugs targeting muscarinic receptors for treating conditions like Parkinson's disease and other movement disorders.