Darenzepine is primarily used in research settings to study its effects on the central nervous system. It acts as a selective antagonist for M1 muscarinic acetylcholine receptors, making it a valuable tool for understanding the role of these receptors in cognitive functions, memory, and neurodegenerative diseases like Alzheimer's. Its application extends to investigating potential therapeutic strategies for conditions involving cholinergic dysfunction. Additionally, Darenzepine is utilized in pharmacological studies to explore its impact on gastrointestinal motility and secretion, providing insights into its potential use in treating related disorders.