mAChR-IN-1 is primarily utilized in pharmacological research to study the function and regulation of muscarinic acetylcholine receptors (mAChRs). It serves as a selective inhibitor, helping researchers understand the role of these receptors in various physiological processes, including neurotransmission, cognitive function, and smooth muscle contraction. Its application is significant in exploring potential therapeutic targets for conditions such as Alzheimer's disease, Parkinson's disease, and other disorders related to cholinergic signaling. Additionally, mAChR-IN-1 is used in preclinical studies to evaluate the efficacy and safety of new drugs targeting mAChRs, contributing to the development of novel treatments for neurological and psychiatric conditions.