Oxotremorine M is primarily used in research settings to study the cholinergic system, particularly in neuroscience. It acts as a muscarinic acetylcholine receptor agonist, making it valuable for investigating the role of these receptors in various physiological and pathological processes. Researchers utilize it to explore conditions like Alzheimer's disease, Parkinson's disease, and other neurodegenerative disorders where cholinergic signaling is impaired. Additionally, it is employed in studies focusing on memory, learning, and cognitive functions, as well as in experiments examining the effects of cholinergic activation on behavior and brain activity. Its ability to stimulate muscarinic receptors also makes it a tool for understanding the mechanisms of acetylcholine in the central and peripheral nervous systems.