VU0463271 is primarily utilized in neuroscience research due to its role as a selective negative allosteric modulator of the M1 muscarinic acetylcholine receptor. It is particularly valuable in studies aimed at understanding the function and regulation of muscarinic receptors in the central nervous system. Researchers employ this compound to investigate its effects on cognitive processes, such as learning and memory, and its potential implications in neurological disorders like Alzheimer's disease and schizophrenia. Additionally, VU0463271 is used in pharmacological experiments to explore the therapeutic potential of modulating M1 receptor activity, offering insights into the development of targeted treatments for various brain-related conditions. Its application extends to preclinical models, where it helps elucidate the complex signaling pathways associated with muscarinic receptor modulation.