Arecaidine but-2-ynyl ester tosylate is primarily utilized in research settings, particularly in the field of neuroscience and pharmacology. It acts as a selective agonist for muscarinic acetylcholine receptors, making it valuable for studying the role of these receptors in the central and peripheral nervous systems. Researchers use this compound to investigate cholinergic signaling pathways, which are crucial for understanding cognitive functions, memory, and neurodegenerative diseases such as Alzheimer's. Additionally, it serves as a tool for exploring the physiological and pathological effects of muscarinic receptor activation, aiding in the development of potential therapeutic agents targeting these receptors. Its specificity and potency make it a key compound in experimental studies focused on receptor pharmacology and neurochemistry.