W-84 dibromide is primarily utilized in biochemical research as a selective antagonist for muscarinic acetylcholine receptors. It is particularly valuable in studies aimed at understanding the role of these receptors in the central and peripheral nervous systems. Researchers use W-84 dibromide to investigate receptor binding properties, signal transduction mechanisms, and the effects of receptor blockade in various physiological and pathological conditions. Its application extends to exploring potential therapeutic targets for disorders such as Alzheimer's disease, Parkinson's disease, and other neurodegenerative conditions. Additionally, W-84 dibromide is employed in pharmacological experiments to differentiate between muscarinic receptor subtypes, aiding in the development of more specific and effective drugs.