KU-0063794 is primarily used in biochemical research to study cellular signaling pathways, particularly those involving mTOR (mechanistic target of rapamycin). It acts as a potent and selective inhibitor of mTORC1 and mTORC2, which are key regulators of cell growth, proliferation, and survival. Researchers utilize this compound to investigate the role of mTOR in various biological processes, including cancer, metabolism, and aging. Its ability to block both mTOR complexes makes it a valuable tool for understanding the complex mechanisms underlying diseases such as cancer, where mTOR signaling is often dysregulated. Additionally, KU-0063794 is employed in preclinical studies to explore potential therapeutic strategies targeting mTOR pathways.