Rheb inhibitor NR1 is primarily utilized in research settings to study the mTOR (mechanistic target of rapamycin) signaling pathway, which plays a critical role in cell growth, proliferation, and survival. By inhibiting Rheb (Ras homolog enriched in brain), this compound effectively suppresses mTORC1 (mTOR complex 1) activation, making it a valuable tool for investigating cellular processes such as autophagy, metabolism, and cancer biology. It is particularly useful in preclinical studies to explore the therapeutic potential of targeting mTOR signaling in diseases like cancer, neurodegenerative disorders, and metabolic syndromes. Additionally, it aids in understanding the molecular mechanisms underlying cellular responses to nutrient availability and stress conditions.