Neutral balsam is widely used in microscopy as a mounting medium for preparing permanent slides. It helps preserve biological specimens by embedding them in a clear, stable medium, ensuring long-term clarity and protection. Its refractive index is close to that of glass, making it ideal for enhancing the visibility of microscopic details. Additionally, it is used in histology and botany for mounting thin tissue sections or plant specimens, providing a durable and transparent finish. Neutral balsam is also employed in the preparation of stained slides, as it does not interfere with the dyes used in staining processes. Its ease of use and effectiveness make it a staple in laboratory settings.