Neutral balsam is widely used in microscopy as a mounting medium for preparing permanent slides. It helps in preserving and protecting biological specimens by embedding them in a clear, stable medium, ensuring long-term visibility under the microscope. Its refractive index is close to that of glass, making it ideal for enhancing the clarity of samples. Additionally, it is used in histology to mount tissue sections, providing a durable and transparent finish. Neutral balsam is also employed in the preparation of botanical specimens, as it maintains the natural colors and structures of plants. Its adhesive properties make it suitable for securing coverslips onto slides without causing distortion or damage to the samples.