Used in research to detect and quantify S100A4 protein expression in tissues and cell lines, particularly in studies related to cancer progression and metastasis. Helps identify tumor cells involved in invasion and migration due to S100A4's role in promoting motility and epithelial-mesenchymal transition. Applied in immunohistochemistry, Western blotting, and flow cytometry to investigate the involvement of S100A4 in various cancers, including breast, colorectal, and pancreatic carcinomas. Also utilized in preclinical studies to validate S100A4 as a therapeutic target and biomarker for metastatic disease.