This chemical plays a crucial role in the modification of 5S ribosomal RNA (rRNA), which is essential for the proper functioning of ribosomes. By modifying 5S rRNA, it ensures the accurate assembly and stability of ribosomes, which are vital for protein synthesis in cells. This process is particularly important in maintaining cellular health and function, as ribosomes are responsible for translating genetic information into proteins. Additionally, the modification of 5S rRNA can influence the efficiency and fidelity of protein synthesis, impacting various biological processes and cellular activities. Its application is significant in molecular biology research, particularly in studies focused on ribosome biogenesis, gene expression, and cellular metabolism. Understanding its role can also provide insights into diseases related to ribosomal dysfunction, such as certain types of cancer and genetic disorders.