RS-1 is widely used in genetic engineering and gene editing research, particularly in enhancing the efficiency of homology-directed repair (HDR) during CRISPR-Cas9 genome editing. It increases the precision of gene modifications by promoting the insertion or correction of specific DNA sequences using a repair template, making it valuable for generating knock-in cell lines and correcting disease-causing mutations. RS-1 is especially useful in stem cell research and functional genomics where accurate genome modifications are critical. Its application improves the yield of desired genetic edits, reducing the need for extensive screening of edited cells.