N-Chloroacetyl-DL-isoleucine is primarily used in the synthesis of peptides and other biologically active compounds. It serves as a key intermediate in the production of modified amino acids, which are essential for developing pharmaceutical drugs and research chemicals. The compound is particularly valuable in the field of medicinal chemistry, where it aids in the creation of peptide-based therapeutics. These therapeutics are often designed to target specific diseases, including cancer and metabolic disorders. Additionally, it is utilized in the study of enzyme mechanisms and protein interactions, providing insights into biochemical pathways. Its role in facilitating the introduction of chloroacetyl groups into molecules makes it a versatile tool in organic synthesis, enabling the development of complex molecular structures with potential therapeutic applications.