D-Norvaline is utilized in biochemical research as a non-proteinogenic amino acid, often serving as a building block in peptide synthesis. It is employed in studies to investigate enzyme specificity and mechanisms, particularly in enzymes that interact with amino acids. Additionally, D-Norvaline is used in the development of chiral catalysts and as a reference compound in analytical chemistry to separate and identify amino acid enantiomers. Its role in metabolic pathway studies also helps in understanding amino acid metabolism and its impact on biological systems.