H-D-Aspartic acid-β-methyl ester is widely used in peptide synthesis as a building block for creating modified peptides. Its methyl ester group enhances stability and solubility, making it valuable in designing peptide-based drugs and bioactive molecules. It is also employed in research to study enzyme mechanisms, particularly those involving aspartic acid residues. Additionally, it serves as a precursor in the synthesis of specialized amino acid derivatives for pharmaceutical and biochemical applications.