N-Formyl-DL-methionine is primarily used in biochemical research and pharmaceutical applications. It serves as a key intermediate in the synthesis of peptides and proteins, particularly in studies involving methionine metabolism. This compound is also utilized in the preparation of modified amino acids, which are essential for investigating enzyme mechanisms and protein functions. Additionally, it finds application in the development of antimicrobial agents, as it plays a role in the formulation of compounds targeting bacterial protein synthesis. Its utility extends to the study of formylation processes in biological systems, contributing to advancements in understanding cellular signaling and metabolic pathways.