Acetyl-L-phenylalanine methyl amide is a protected dipeptide analog primarily used as a model substrate in biochemical assays for proteolytic enzymes, such as carboxypeptidases and endopeptidases. It enables studies of enzyme kinetics, substrate specificity, hydrolysis mechanisms, and inhibitor screening. Valuable in pharmaceutical research for developing protease inhibitors targeting neurological and metabolic disorders. Its chiral structure also supports applications in asymmetric synthesis and stereochemical studies in organic chemistry.