O-Methyl-L-threonine finds application in the field of biochemistry and medicinal chemistry as a building block for the synthesis of more complex molecules. It is used in the development of peptide-based drugs and bioactive compounds, where its unique structure can influence the biological activity and stability of the final product. Additionally, it serves as a precursor in the synthesis of specialized amino acids and analogs, which are valuable in studying enzyme mechanisms and protein interactions. Its role in research extends to the exploration of metabolic pathways and the design of inhibitors for specific biochemical targets.