Sodium 1-heptanesulfonate is primarily used as an ion-pairing agent in high-performance liquid chromatography (HPLC) to improve the separation and detection of hydrophobic compounds. It is particularly effective in the analysis of organic molecules, such as pharmaceuticals and biomolecules, by enhancing their retention time on reversed-phase columns. Additionally, it serves as a surfactant in various industrial applications, including detergents and cleaning agents, due to its ability to reduce surface tension and improve wetting properties. In biochemical research, it is utilized in protein purification processes to aid in the solubilization of membrane proteins. Its compatibility with aqueous solutions makes it a versatile additive in formulations requiring stability and solubility.