MitoTEMPO hydrate is widely used in research for its ability to target and reduce mitochondrial oxidative stress. It acts as a mitochondria-specific antioxidant by scavenging reactive oxygen species (ROS) within the mitochondria, making it valuable in studying diseases linked to oxidative damage. It is commonly applied in cellular and animal models of neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions to assess the role of mitochondrial ROS in disease progression. Researchers also use it to protect cells during ischemia-reperfusion injury, where oxidative stress significantly contributes to tissue damage. Its targeted action allows for more precise investigation of mitochondrial function and dysfunction in various pathological states.