Mito-TEMPO is widely used in research to target and reduce mitochondrial oxidative stress. It accumulates within mitochondria due to its lipophilic cationic structure, where it acts as a potent scavenger of reactive oxygen species (ROS), particularly superoxide radicals. This makes it a valuable tool in studies related to cellular aging, neurodegenerative diseases, ischemia-reperfusion injury, and inflammatory conditions. By protecting mitochondrial function and preserving cellular energy production, Mito-TEMPO helps researchers investigate the role of mitochondrial ROS in disease progression and evaluate potential therapeutic strategies. It is commonly applied in cell culture models and animal studies to assess the impact of oxidative stress mitigation on cell survival, inflammation, and organ function.