Mdivi-1 is primarily used in research settings to study mitochondrial dynamics. It functions as a selective inhibitor of mitochondrial fission by targeting the protein Drp1 (dynamin-related protein 1). This makes it a valuable tool for investigating the role of mitochondrial fission in various cellular processes and diseases. Researchers utilize Mdivi-1 to explore its effects on conditions such as neurodegenerative diseases, ischemia-reperfusion injury, and cancer, where mitochondrial dysfunction plays a critical role. Additionally, it helps in understanding the balance between mitochondrial fission and fusion, which is essential for maintaining cellular health and energy production. Its application extends to preclinical studies aimed at developing therapeutic strategies targeting mitochondrial dynamics.