VBIT-12 is primarily used in research settings to study its effects on mitochondrial function and cellular metabolism. It has shown potential in modulating the activity of the mitochondrial permeability transition pore (mPTP), which plays a critical role in cell survival and death pathways. This makes it a valuable tool for investigating conditions related to mitochondrial dysfunction, such as neurodegenerative diseases, cardiovascular disorders, and ischemia-reperfusion injury. Additionally, VBIT-12 is explored for its potential therapeutic applications in protecting cells from oxidative stress and apoptosis, offering insights into developing treatments for diseases linked to mitochondrial impairment.