S3QEL-2 is primarily utilized in research settings as a selective inhibitor of mitochondrial complex III, specifically targeting the Qo site. Its application is significant in studying cellular respiration and the role of reactive oxygen species (ROS) in various biological processes. Researchers employ S3QEL-2 to investigate the impact of mitochondrial dysfunction on diseases such as neurodegenerative disorders, cancer, and cardiovascular conditions. By modulating mitochondrial activity, it helps elucidate the mechanisms of oxidative stress and its contribution to cellular damage or signaling pathways. Additionally, S3QEL-2 is used in pharmacological studies to explore potential therapeutic strategies aimed at mitigating ROS-related damage in pathological conditions. Its specificity makes it a valuable tool for dissecting the complex interplay between mitochondrial function and cellular health.