Nocodazole is widely used in biological research as a microtubule-depolymerizing agent. It is particularly valuable in cell biology studies to arrest cells in the mitotic phase of the cell cycle by disrupting microtubule formation. This property makes it a useful tool for investigating cell division, chromosome segregation, spindle assembly, and generating polyploid cells. Additionally, it is employed in studies of intracellular transport and cytoskeleton dynamics, as it inhibits vesicular trafficking by destabilizing microtubules. Nocodazole is also used in cancer research to study the effects of microtubule disruption on tumor cell proliferation and apoptosis. Its reversible effects allow researchers to wash it out and observe cellular recovery processes.