Tubulin Inhibitor 8 is primarily used in research settings to study the mechanisms of cell division, particularly the role of tubulin in mitosis. It is employed to disrupt microtubule formation, which is essential for the proper segregation of chromosomes during cell division. This makes it a valuable tool for investigating cancer biology, as many cancer therapies target microtubule dynamics to inhibit the proliferation of cancer cells. Additionally, it is used in pharmacological studies to explore potential anti-cancer agents and to understand the resistance mechanisms that some cancers develop against microtubule-targeting drugs. Its application extends to the study of neurodegenerative diseases, where microtubule dysfunction plays a critical role, aiding in the development of therapeutic strategies.