TB Medium is widely used in microbiology for the cultivation of bacterial cultures, particularly Escherichia coli, in high-density fermentation processes. It supports rapid cell growth and high protein yields, making it ideal for recombinant protein expression in research and industrial applications. Due to its rich composition, including peptones, glycerol, and phosphate buffer, TB Medium maintains stable pH and provides essential nutrients during prolonged incubation, which is critical for large-scale protein production. It is commonly used in molecular biology for plasmid preparation and protein expression systems requiring high cell density.