SR3335 is primarily used in research settings as a modulator of circadian rhythm pathways. It acts as an inhibitor of the REV-ERBα and REV-ERBβ receptors, which play key roles in regulating biological clocks, metabolism, and inflammatory responses. Due to this activity, SR3335 is employed in studies exploring metabolic disorders such as diabetes and obesity, where circadian misalignment contributes to disease progression. It has also shown potential in neurodegenerative and mood disorder research, where disruption of circadian rhythms is a common feature. Additionally, SR3335 is used to investigate the role of REV-ERB signaling in inflammation and immune function, making it a valuable tool in immunology and chronobiology research. Its ability to penetrate cells and modulate gene expression related to circadian cycles makes it suitable for in vitro and in vivo experimental models.