Used primarily in biomedical research to induce diabetes in laboratory animals, particularly rodents, by selectively destroying insulin-producing beta cells in the pancreas. This makes it a valuable tool for studying diabetes pathophysiology, testing antidiabetic drugs, and evaluating the effects of various treatments on glucose metabolism. Due to its toxic nature and specific mechanism, it is not used in clinical medicine or industrial applications. Research involving alloxan tetrahydrate requires careful handling and ethical oversight due to its use in animal models.